This powerful, state-of-the-art system can perform confocal 4D imaging that is optimized for simultaneous spectral detection of multiple labels with high spatial resolution.
- Accessibility
Technical Specifications
Microscope
- Laser scanning confocal and Airyscan 2 imaging modalities
- Photobleaching and fluorescence correlation spectroscopy
- A range of imaging and contrast techniques, including DIC and widefield fluorescence
- Nonlinear optical imaging capability (TPEF/SHG)
- Lateral resolution of 120 nm and Axial resolution of 350 nm at 488 nm
- 6 virtually simultaneous imaging channels with freely selectable emission bands
- Spectral unmixing of up to 10 fluorophores
Environmental Control
- Attached incubator for live cell imaging
- Heated slide/sample holders
Objectives & Laser Lines
- Wide range of objectives (2.5x/0.85, 10x/0.45, 20x/0.5, 40x/1.3, 63x/1.4) with different magnifications and immersion media
- Solid state diode laser lines: 405-445-488-514-561-639 nm
- Ti-Sapphire femtosecond tunable laser (680-1080 nm, centered at 800 nm for SHG/TPEF imaging)
Software and Image Analysis
- ZEN Blue 7.4
- Separate workstation for image analysis
Interested in
learning more?
We always look forward to hearing from motivated potential collaborators, partners, and trainees.
Contact Us Newsletter Form
Interested in Exploring More?
The Team
We are a group of experts from different disciplines working together to tackle one of the biggest challenges of modern times: the characterization and recreation of tissue microenvironments.
Research
We seek answers to questions related to tissue homeostasis and disease. New technologies resulting from our work will facilitate diagnosis and treatment of many chronic diseases.
Infrastructure
Our state-of-the-art microscopy and analytical techniques allow us to analyze the complex biochemical and biophysical processes that occur in tissue microenvironments.
Training
By providing research opportunities and developing educational programs for the larger community, we are helping Ontario and, indeed, all of Canada build expertise in emerging but increasingly important areas of research, including tissue engineering and imaging.
