WiTec Alpha300 with SHG

This modular Raman imaging system fulfills three crucial tasks simultaneously, providing the best spatial and spectral resolution along with peerless sensitivity and unmatched speed.


Photos courtesy of: raman.oxinst.com

Technical Specifications


  • Spectral resolution down to 0.1 relative wavenumbers (@633 nm excitation)
  • Lateral, diffraction-limited spatial resolution down to ~200 nm
  • Focus stabilization to compensate for thermal and mechanical variations during long-term measurements 
  • Fast Raman Imaging with motorized or piezo-driven scanning stages 
  • 3D images and depth profiles with motorized or piezo-driven scanning stages

Environmental Control

  • Acoustic and vibrational noise enclosure
  • Airtight option to allow control of the gas phase during experiments

Objectives & Laser Lines

  • 10x, Zeiss EC Epiplan-Neofluar / NA 0.25 / WD 9.3 mm
  • 50x, Zeiss EC Epiplan-Neofluar DIC / NA 0.8 / WD 0.58 mm
  • 100x, Zeiss EC Epiplan-Neofluar DIC / NA 0.9 / WD 1.00 mm 
  • 532 nm, 30 mW power at laser output 
  • 1064 nm, >200 mW average power output, Pulse width <10ps


  • Integrated software interface for user-friendly measurement control
  • Precise and absolute laser power adjustment for up to four lasers 
  • Automated light intensity adjustment
  • High-speed overload protection for photon-counting detectors 
  • Two built-in fully digital lock-in amplifiers (real amplitude and real phase up to 500 KHz)
  • Real-time rotation matrix and real-time translation for XYZ scans

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The Team

We are a group of experts from different disciplines working together to tackle one of the biggest challenges of modern times: the characterization and recreation of tissue microenvironments.


We seek answers to questions related to tissue homeostasis and disease. New technologies resulting from our work will facilitate diagnosis and treatment of many chronic diseases.


Our state-of-the-art microscopy and analytical techniques allow us to analyze the complex biochemical and biophysical processes that occur in tissue microenvironments.


By providing research opportunities and developing educational programs for the larger community, we are helping Ontario and, indeed, all of Canada build expertise in emerging but increasingly important areas of research, including tissue engineering and imaging.